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Incretin action of GIP is dramatically reduced in the presence of diabetes, as demonstrated by the evidence that glucose-dependent insulin secretion is not potentiated by GIP administration in subjects with diabetes [33, 34]

Formulation differences: Compounded tirzepatide may include additional ingredients (B12, L-carnitine, glycine) not present in Zepbound

After a 15 min centrifugation at 14000 g , the supernatants were diluted 10 times, and 100 L was transferred to sample vials for further detection

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Plates were then incubated for a further 24 h at 37C and 150 rpm