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Whole-cell patch-clamp recordings were performed on mRFP--actin-transfected hippocampal neurons to measure miniature excitatory postsynaptic currents (mEPSCs), reflecting synaptic activity [2]

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Philippe Halban, University of Geneva, Switzerland) were maintained in DMEM at 25 mM d-glucose supplemented with 15% FBS, 50 M -mercaptoethanol, and 1% penicillin/streptomycin 22