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This pigmentation effect: Can confound behavioral assays that rely on visual observation of coat color (e.g., agouti gene expression studies) Serves as a visual pharmacodynamic marker confirming MC1R engagement and compound activity Is relevant to research on melanoma biology, melanocyte function, and photoprotection May complicate blinding in non-pharmacological behavioral studies PT-141's minimal MC1R activity eliminates this pigmentation confound, making it the preferred compound for CNS-focused research where skin color changes would be undesirable

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