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For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, Cat# H-3300-250)

An earlier short-term randomized trial did not find meaningful changes in several oxidative-stress markers after oral glutathione

Its level decreases with age (especially from the age of 45-50) and as the body's defence needs increase (immunity, liver detoxification, antioxidant defences...)

Cell cycle analysis The position of the cells in the cell cycle was determined by flow cytometric analysis of the DNA content using propidium iodide

In summary, vitamin B12 is an essential nutrient that helps create new red blood cells, maintain nerves, produce DNA, and enable cell division