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When GLP-1 binds to GLP-1R on the surface of -cells, cAMP-dependent PKA is activated and subsequently activates the downstream signal PI3K, which inhibits apoptosis in islet -cells by blocking the expression of the pro-apoptotic protein Bax (Li et al., 2021) (Figure 4)
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After TMB substrate solution is added, only those wells that contain Human SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color
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