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[0032] The present invention describes two methods for the synthesis of Semaglutide: Method 1: Sequential coupling [0033] The route for sequential coupling is: Method 2: Sequential coupling + fragmental synthesis [0034] The schematic diagram for analysis of sequential coupling + fragmental synthesis is: [0035] The synthesis route of the present invention comprises the following steps: 1) selecting an appropriate carrier and attaching Fmoc-Gly-OH to the resin by solid phase synthesis

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In study 2, linagliptin (3 mg/kg/day, s.c.) was administered alone or in combination with an Y2R-selective PYY 336 analogue (compound 38

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