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Characterization of procaspase-1 cleavage in mammalian cells To determine whether X(PC) can cleave full-length procaspase-1 in a cellular context, we transfected hemagglutinin (HA)-tagged procaspase-1-Cys285Ala into HEK293T cells and incubated lysates with decreasing doses of purified proteases (Extended Data Fig

Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37C

It represents a minimal loss that does not significantly affect your dose

Gupta D, Khan S, Islam M, Malik BH, Rutkofsky IH