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We also applied a series of complementary proximity-based techniques based on both BRET and FRET to monitor GLP-1R redistribution between the plasma membrane and different endosomal compartments

All blood samples were immediately centrifuged at 1,600 x g for 15 min at 4C and the resulting plasma subsequently shock frozen in liquid nitrogen

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If it appears thick, gel-like, or viscous in ways that differ from when you first received it, the protein may have aggregated into a higher-order structure that changes the solution physical properties