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Front Microbiol 13:933550
The following factors were used: 50 ng/mL recombinant EGF, 500 nM A83-01 (Biogems), 100 ng/ mL recombinant Noggin (Peprotech), 10 M Y-27632 (Abmole), 500ng/mL R-spondin-1(Peprotech), 10 mM nicotinamide (Sigma), and 10 M SB202190 (Sigma)
To the reaction mixture, containing deprotected pentapeptide hydrochloride and Pd(OH) 2 , was added a solution of Z-Asp( O t Bu)-OPfp (26.3 g) in THF (150 mL), and the resulting mixture was stirred for 1 h at r.t
Analysis of leukocyte and sperm telomere length in oligozoospermic men
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