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Kaiserlian) were cultured in high-glucose DMEM (Lonza, BE12-604F), 10% FCS (Biochrome, S0115), HEPES (10 mM, Biochrome, L1613), non-essential amino acids (1 mM, Gibco, 11140-035), 100U/ml penicillin and 100 g/ml streptomycin (1% Biochrome, 0257 F)
A randomized clinical trial in OA patients of PF-04457845, a potent FAAH (fatty acid amide hydrolase with a prominent role in the hydrolysis of endocannabinoids) inhibitor, indicated no significant difference in analgesia compared to placebo (NCT00981357) (46)
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Materials and methods Animals A total of 98 female Wistar rats aged 68 weeks and weighing 190210 g were selected for in vivo experiments, and 8 pregnant Wistar rats at gestational days 1214 were used for in vitro experiments
Even if its safe in small doses, we have no data about what happens at higher doses
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