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After reconstitution, formation of visible particulates, haziness, or a significant drop in measured peptide concentration by UV spectrophotometry at 280 nm compared to the expected value suggests aggregation and precipitation of degraded species
N., Ali, B
Protein bands were visualized using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, Inc.) and imaged using the ChemiDoc XRS+ Imaging System (Bio-Rad Laboratories, Inc.)
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