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We therefore sought a strategy to reduce the scale of the library to remain within the quantitative throughput limits of our assay (up to ~40,000 substrates), while still assaying a full repertoire of sequences likely to be cleaved by our evolved proteases (Extended Data Fig

ATP generation via glycolysis and mitochondrial respiration, previously believed to be physically uncoupled, engage in crosstalk to maximize sperm function, competition and overall fertility

Allow the lyophilized powder to reach room temperature before opening the vial

Fan Z, Wirth AK, Chen D, Wruck CJ, Rauh M, Buchfelder M, et al