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Global and mitochondrial PKA assays INS-1 832/3 cells were seeded onto 35-mm glass-bottom dishes (MatTek) and transfected with enhanced excitation-ratiometric PKA biosensor pcDNA3.1(+)-ExRai-AKAR2 (for global PKA) or pcDNA3-mitoExRai-AKAR2 (for mitochondrial PKA) 26

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However, the detailed mechanism underlying gut microbiota-mediated endocrine signaling in the modulation of locomotion is still unclear