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HEK293T cells were transfected with 1 g lentiCRISPRv2 containing the sgRNA insert, 0.67 g psPAX2 (Addgene, 12260) and 0.33 g pVSVg (Addgene, 8454) using 6 g polyethylenimine (Sigma-Aldrich, 919012) in Opti-MEM (Thermo Fisher, 31985062)
10.1039/c2cc30677h 43 HallA.NelsonK.PooleL
Modulation of Neurotransmitter Systems DSIP does not appear to act as a direct ligand for a single neurotransmitter receptor (like a key in a lock)
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