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The labeled protein concentrations were adjusted to 100 nM based on fluorescence intensity measured during pretest scans, ensuring signal quality and uniformity across all three Syn constructs for direct comparison of binding affinities
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doi: 10.1111/ppa.12893 49 Mndez-LpezE.DonaireL.GoslvezB.Daz-VivancosP.Snchez-PinaM
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