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In every case we've tracked, this coincides with either (a) return to baseline-poor sleep habits, (b) increased stress/cortisol load, or (c) dietary regression

Plates were incubated at 37C for 2 h and then fluorescence was measured with excitation at 360 nm and emission at 460 nm using a ClarioStar Monochromator Microplate Reader (BMG LABTECH, Ortenberg, Germany)

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Bright field mode was used to detect for the development of blue color under a Zeiss Axioscope microscope equipped with a Zeiss HRC microscope camera (200 total magnification)

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