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These tissue sections were washed with phosphate-buffered saline (PBS) and incubated overnight with anti-GLP-1R (1:200, NBP1-97308, Novus Biologicals, USA) at 4 C
doi:10.1098/rsbm.1982.0026
The expanded Enterobacteriaceae release LPS, activating the TLR4/MyD88 axis to upregulate epithelial NOX1 expression, thereby driving secondary ROS bursts that amplify epithelial barrier disruption and inflammatory cascades [404]
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