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when oxidative stress depletes GSH faster than it can be regenerated, the ratio collapses, signaling cellular redox imbalance
HPLCMS analysis of flavonoids HPLCMS analyses of samples from in vitro reactions or S
D.CarvalhoA
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Cells were subsequently blocked by 2% BSA in PBS-T (0.1% Tween 20 in PBS) for 1 h at room temperature, incubated with the primary antibody Anti-Rheb (Santa Cruz Biotechnology Cat# sc-271509, RRID: AB_10659102, 1:500) overnight at 4 C in blocking buffer, then washed with PBS-T three times and incubated with Alexa-555-conjugated secondary antibody (Invitrogen # A-31570, RRID:AB_2536180, 1: 1000) and DAPI (1 g/ml) in blocking buffer for 1 h at room temperature with protection from light
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