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In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out
Immunobiol 218:116674
Prog Neuro-Psychoph
To view a copy of this license, visit About this article Cite this article Kelly, R.A., Leedale, J., Calleja, D
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