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The digest was filtered through a 70-m mesh and centrifuged using a 28.7% Nycodenz gradient (Nycomed PharmaAS, Oslo, Norway)

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The differential equation for the concentration of cytosolic cysteine (Cys) is straightforward: The first term on the right is the production of cysteine from cystathionine (Cysta) by CGTL and the second term is the import of cysteine into the cell, which depends on the concentration of cysteine in the blood ([bCys])

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The glutathione solution is slowly administered into the bloodstream via the IV drip