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How It Is Studied Pharmacological characterization of sema at the GLP-1 receptor uses cAMP accumulation assays in GLP-1R-expressing HEK293 cells, radioligand competition binding with [125I]-GLP-1 or [3H]-exendin-4, and beta-arrestin recruitment BRET assays to determine binding affinity, efficacy, and biased agonism profiles

The National Cancer Institute USA explored the feasibility of a weight loss intervention for breast cancer prevention clinical trial [26], and an endpoint of survivorship, rather than incidence was deemed most feasible and was pursued [26, 27]

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