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The sample was further purified via size exclusion using a HiLoad 26/60 Superdex 75 pg gel filtration column and equilibration buffer (20 mM Tris pH 8.0, 150 mM NaCl, 5 mM -mercaptoethanol)

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Signs of contaminated product Contaminated or degraded peptides can cause effects unrelated to AOD 9604 itself: Fever or chills following injection, suggesting possible bacterial contamination Severe injection site reactions with significant swelling, heat, or spreading redness Systemic illness symptoms that seem disproportionate to what the peptide should cause Effects that seem completely different from expected AOD 9604 effects If contamination is suspected, stop using that vial immediately