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2020;31(2):233-49.e4
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Tandem mass spectrometric sequencing An aliquot of the lyophilized venom fraction containing approximately 2 nmol was reduced with 5 mM of Tris(2-carboxyethyl)phosphine hydrochloride (TCEP) for 15 min at 55 C and alkylated with 20 mM iodoacetamide (IAA) for 30 min at room temperature
Significant weight loss results are typically observed within the first two months of treatment
Since it gained popularity as a treatment for obesity, it led to the off-label prescribing of the drug for weight loss
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