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Loren Pickart isolated it from human plasma in 1973 while investigating why blood plasma from younger donors stimulated regeneration in older liver tissue more effectively than plasma from older donors
10.1038/nature23876 Nature
Nucleic Acids Research, 42(W1), W252W258
Excess DTT and imidazole were removed on a PD-10 desalting column (Merck), and the reduced proteins were eluted with 3.5 mL ice-cold assay buffer containing 100 mM Tris/HCl, 1 mM EDTA, pH 8.0
For example, the main clinical manifestation of OSF is a burning sensation and intolerance to spices, and as the disease progresses, patients experience reduction in mouth opening, which not only causes pain, poor oral hygiene and impaired chewing and swallowing, but also affects patients speaking and appearance, causing psychological issues and limitations in social activities (Peng et al., 2020)
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