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In a 6 yr old leukemia patient receiving pentostatin 0.25 mg/kg daily for 3 successive days by direct iv injection, serum and CSF (via lumbar puncture) pentostatin concentrations 4 hr after the initial dose were approximately 147 and 19 ng/ml, respectively, using an enzyme-inhibition titration assay

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The brain is particularly vulnerable to oxidative stress due to its high metabolic rate and relatively limited antioxidant defences

Proteins were precipitated with 160 g of Hydroxyl beads (MR-HYX010) using the Protein Aggregation Capture (PAC) method, with precipitation carried out in 60% ethanol and three washes in 80% ethanol 65