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The cell suspensions were washed twice with ice-cold PBS before staining using an Annexin V/PI staining kit (Alexa Fluor 488 annexin V/Dead Cell Apoptosis Kit)
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2B) was 0.77 at 3 months, 0.65 at 6 months, 0.38 at 9 months, and 0.27 at 12 months in not treated subjects
