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Nanoparticles were diluted in phenol red-free DMEM/HAMs F-12 medium (Gibco, Life Technologies, Carlsbad, CA, USA) supplemented with 5% FBS at a final concentration of 100 g/ml and added to the donor compartment (0.2 ml)

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In addition, the blood pressure of tails was not significantly different between the control and BPC 157 groups either 5,6

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In a recently published report, autophagic flux restoration ameliorated cell senescence 39