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To confirm whether an increase in UCK2 activity as a result of pyrimidine stress was the driving force behind mitochondrial biogenesis signalling, we knocked down UCK2 in cells using siRNA (Fig
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21, 22 Further, ROS production has been demonstrated to occur not only in the endothelial cell layer but also within the media and adventitia, all of which may impair NO signaling within vascular tissues
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