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The authors attributed the increased risk to activation of cytochrome P450 2E1 by alcohol and depletion of glutathione stores by starvation or alcohol abuse

Sample pre-treatment: Glutathione standard (GSH powder) was centrifuged on a low-speed centrifuge to ensure that the powder was at the bottom of the tube

Starting at 250-300mcg regardless of body weight, then adjusting based on response, often works better than jumping to weight-based higher doses

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Injection site (abdomen, thigh, or upper arm) may cause minor variations in absorption rate, but these differences are not clinically significant