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The apo and holo systems were solvated with Tip4p-e water and neutralized by adding Na + and Cl - ions to achieve a concentration of 0.15 M

Recording the reconstitution date, diluent used, calculated concentration, and storage location for every vial makes it possible to audit exactly how far into its stability window a given sample sits at the time it is used in an assay

Outline of the standardized, stepwise approach to performing and interpreting the methacholine challenge test