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In addition, when an activity test was performed for several hours with HQ as a sole substrate in comparison to a control without enzyme, low activity was visually observed, even without the addition of H 2 O 2 or L-dopa, and a light brown color was detected after 3 hours of incubation (the control remained colorless)

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GLUTag cell viability were not affected by the buffer conditions or additives used in this study (Supplementary Figure S1) and GLP-1 secretion under basal buffer conditions was not affected by the presence (460 102 pg GLP-1, n = 6) or absence (505 17 pg GLP-1/ml, n = 6) of Mg 2+

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