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Continuous supplementation or maintenance is often needed to retain the effect
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The fluorescence intensities of Cell Tracker Blue and CM-H 2 DCFDA were assessed via fluorescence microscopy (Eclipse 80i, Nikon Instruments Europe, Amsterdam, the Netherlands) at 20 magnification with an Ex filter of 365/28 nm, a DM of 405 nm, an Em filter of 445/50 nm, an Ex filter of 475/30 nm, a DM of 405 nm and an Em filter of 530/40 nm
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Fixed-dose combination means less flexibility in dosing
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