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Analysis of CE molecular species was performed as previously described 59

Nutrition 65:5059

AOD-9604 (Anti-Obesity Drug 9604) is a 16-amino-acid synthetic peptide based on residues 176-191 of the C-terminal region of human growth hormone, with the N-terminal phenylalanine replaced by tyrosine to improve stability

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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution