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Methods: Healthy human PCLS were cultured for up to 96 hours in either GFIPO medium (Williams E Medium supplemented with Glucose (36 mM), Fructose (5 mM), Insulin (1 nM), Palmitic acid (0.24 mM) and Oleic acid (0.48 mM)) to mimic NAFLD-inducing conditions, or WEGG medium (Williams E Medium supplemented with Glucose (25 mM)) as a control
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Fortier L, Mohammed HO, Lust G, et al
SGLT-2 inhibitors and thiazolidinediones may also be used concurrently, though patients should be monitored for any unexpected interactions or adverse effects
Cell plates were transferred to a LUMIstar plate reader (BMG Labtech) at 30 C for BRET measurements
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