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Clinical guidance for intramuscular injections points to a 22 to 25 gauge needle, given at a 90-degree angle, with the length chosen so it reaches muscle without going too deep

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Western blot analysis Western blotting was performed as previously described via the following antibodies: FBXO22, BACH1, KLF4, LKB1, SNAL1, PTEN, KDM4A, BAG3, and GAPDH (all from Proteintech, Rosemout, IL)

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