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doi:10.1016/S1262-3636(08)73398-6 Secher A, Jelsing J, Baquero AF, Hecksher-Srensen J, Cowley MA, Dalbge LS, Hansen G, Grove KL, Pyke C, Raun K, Schffer L, Tang-Christensen M, Verma S, Witgen BM, Vrang N, Bjerre Knudsen L
The supernatant was collected from centrifugation at 12,000 g for 15 min and read the fluorescence energy at excitation (440 nm) and emission (640 nm) by spectrofluorometer (Hitachi F-7100) according to [32]
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This protocol is often used to supplement other services like testosterone replacement, medical weight management, and the GLP-1 program
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