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Description

Briefly, jejunum (10 mg) was homogenized in DNA lysis buffer (pH 8.0) [100 mM TrisHCl (pH 8.0), 5 mM EDTA, 0.2% SDS, 200 mM NaCl] and incubated with proteinase K solution (Sigma) at 55 C overnight

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For someone with a maintenance level around 2,200 calories, this means eating approximately 1,650-1,760 calories

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