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HUVEC cells were stained with 0.5 M Calcein-AM and the fluorescence was detected by fluorescence microscope, and quantitative analysis of each image was performed using Image J 1.53t software
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Less is more: Nutrient limitation induces cross-talk of nutrient sensing pathways with [PMID: 27683589] Laboratory literature summary: Less is more: Nutrient limitation induces cross-talk of nutrient sensing pathways with NAD(+) homeostasis and contributes to longevity
Transition to Weekly Administration After the first phase, shots are given weekly
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