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Mechanism of Action Enzymatic Protection Mechanism : The phenylacetyl group acts as a decoy substrate for glutathione-degrading enzymes, redirecting their activity away from glutathione itself and toward cleaving the protective phenylacetyl modification
These findings guide clinical practice while highlighting research priorities to fully establish NACs therapeutic potential in respiratory medicine
doi: 10.1016/j.celrep.2014.10.032 68 GrassetEPuelACharpentierJColletXChristensenJETercFet al
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The iQuant software was utilized to analyze the labeled peptides with isobaric tags quantitatively, and the iTRAQ data analyses were performed by BGI Genomics, Shenzhen, China ( 2.7 Polysome fractionation by sucrose gradients As described ( 2.8 mRNA stability assay As described ( 2.9 RNA-IP and RIP-seq The cells were lysed and immunoprecipitated using FXR1 antibody, with mouse IgG as a negative control
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