Cell culture was performed under aseptic conditions in Class II biosafety cabinets and incubation took place at 37 o C and 5% CO 2 in a humidified incubator
[2] An important caveat applies to all of this work: these studies measure DSIP-like immunoreactivity (DSIP-LI) using antisera, which detects material that cross-reacts with the antibody
Secondary outcome measurements included reduction in body weight from baseline by at least 5% or 10% by week 26, changes in waist circumference, total cholesterol, HDL, LDL, VLDL, and triglycerides, and levels of different glycemic variables including HbA1C, fasting plasma glucose, fasting insulin, insulin resistance, and the functioning of beta-cells
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