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As more researchers develop an interest in 5-amino-1MQ, clear dosing guidelines could greatly facilitate its application in experimental settings

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[30] [31] [32] The first and rate-limiting step in the AAA pathway is the condensation reaction between acetyl-CoA and ketoglutarate catalysed by homocitrate-synthase (HCS) (E.C 2.3.3.14) to give the intermediate homocitrylCoA, which is hydrolysed by the same enzyme to produce homocitrate

PMID: 24828499

In native form, IGF-1 is tightly regulated by these proteins, limiting free circulating activity