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The culture medium was changed every 4872 hours, and subculturing was performed when the cell monolayer reached 80%90% confluence
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The aortas were then incubated in freshly prepared SA--Gal staining solution at pH 6.0 overnight at 37 C, according to the manufacturers protocol
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The column was equilibrated with a minimum of 5 injections (50 l) of sample diluent buffer until a stable baseline was obtained
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