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This is the route used in every animal study demonstrating GHK-Cu's effects beyond the skin
For peripheral nerve analysis, a 5-mm-long piece of tail 15 mm distal from the tail base was collected from each sacrificed animal, fixed in 4% formaldehyde in phosphate buffer (pH 7.4), decalcinated, and impregnated with 1% osmium tetroxide for a few days
with 10 mL, 5 mg/mL
Problems arise when nitric oxide production decreases or when the molecule gets broken down too quickly
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