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Once set, plugs were dehydrated through a serial ethanol gradient and HistoChoice (Sigma) before paraffin embedding with the Histoembedder station (Leica) and cutting to 1 m sections with a Leica Jung RM2035 microtome

It has been demonstrated that, together with the regulation of CPT1 by malonyl-CoA, acetylation of CACT plays a crucial role in the control of the influx of fatty acyl units into mitochondria 57