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Extreme restriction is unnecessary and often counterproductive, causing metabolic slowdown that persists beyond the protocol
Based on preclinical (animal and cellular) data only, inhibition of NNMT by 5-Amino-1MQ is theorised to: Raise intracellular NAD+ levels Activate SIRT1 and other sirtuins (proteins linked to metabolic regulation) Reduce fat cell (adipocyte) size and proliferation Improve insulin sensitivity These effects have been demonstrated in mouse models (Neelakantan et al., 2019), but robust human clinical trial data are currently absent
However, this amount may be slightly higher for certain groups, including people who are pregnant or breastfeeding
Where results are borderline, measurement of methylmalonic acid (MMA) or holotranscobalamin (active B12) may help clarify the picture, as recommended in NICE CKS guidance on B12 and folate deficiency anaemia
Not all B12 injections are equal
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