Purified mRNA was fragmented into small pieces with fragment buffer and reversed into cDNA
Although it is an intermolecular cross-link in a strict sense, a strong protein dimerization, as seen for OPRT, increases the effective concentration of reacting thiols by positioning them in a close spatial proximity
As erythrocytes lack intracellular organelles, their metabolism is restricted to well-known cytosolic biochemistry: glycolysis, the pentose phosphate pathway (PPP), redox metabolism, oxygen metabolism, purine/nucleoside metabolism, and membrane transport
Glutathione S-transferases (GSTs) are the enzymes expressed in the cytosol, microsomes, and mitochondria and catalyze glutathione conjugation to electrophilic compounds, not only produced from exogenous xenobiotics by biotransformation but also arise from endogenous substances
Effervescent form allows for better absorption and digestive ease