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BAC water is good for 28 days once opened, and until the expiration date printed on the label while it stays sealed, typically 2 to 3 years from manufacture
Cells were mixed with transfection reagents and plated at 13,000 cells per well into 96-well Greiner CELLSTAR white-walled plates (Sigma-Aldrich), and assays were performed 48 h later
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