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After washing several times in 0.01 M PBS, the samples were treated with anti-rabbit IgG antibodies (Thermo Fisher Scientific) in 0.01 M PBS at 22 2 C for 1 h

Mice were tested for social interaction (SI) 24 h after the last social defeat by first allowing 2.5 min for the test mouse to explore an arena containing a plexiglass wire mesh cage centered against one wall of the arena (target absent)

That way you will be able to monitor the effectiveness of your treatment plan and hard work while educating and empowering yourself along the way

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