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(2019) Serum gamma-glutamyltransferase and the overall survival of metastatic pancreatic cancer, BMC Cancer , 19 , 1020, doi: Huang, Z
sgRNA constructs and gene expressing codon optimized (OPT) plasmid constructs sgRNA control and constructs against CBS and L2HGDH were designed targeting GTATTACTGATATTGGTGGG (sgControl) (#230080, Addgene), GGAGCAGACAACCTACGAGG (sg CBS -1) (#230081, Addgene), TGGCAGTGCTGGCAGCACGG (sg CBS -2) (#230082, Addgene), GATATAGTCATCGTTGGTGG (sg L2HGDH -1) (#230083, Addgene), and CACCAGACTGGACATAACAG (sg L2HGDH -2) (#230084, Addgene) and constructed in lentiCRISPR v2-Blast (#83480, Addgene) for Blasticidin selection at a final concentration of 5 g/ml
Extracted proteins were removed from the dialysis casing into 1.5-ml Eppendorf tubes, and concentration was measured with Pierce BCA Protein Assay Kit (23225
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