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Boese AC, et al
Dramatic increase in fine lines or skin laxity
Cells were seeded into 35 mm dishes (Corning) or 24-well plates (Corning) and allow to grow to confluence in DMEM/F12 medium (Invitrogen 31330038) containing antimycotic/antibiotic (Sigma A5955) and heat-inactivated serum (Gibco 10500064)
Lifespan extension and delayed immune and collagen aging in mutant mice with defects in growth hormone production
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